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Volume 61, issue 4 | Copyright
Arch. Anim. Breed., 61, 387-394, 2018
https://doi.org/10.5194/aab-61-387-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Original study 12 Oct 2018

Original study | 12 Oct 2018

Development of amplified fragment length polymorphism (AFLP) markers for the identification of Cholistani cattle

Muhammad Haseeb Malik5,*, Muhammad Moaeen-ud-Din1,2,*, Ghulam Bilal2, Abdul Ghaffar3, Raja Danish Muner2, Ghazala Kaukab Raja5, and Waqas Ahmad Khan4 Muhammad Haseeb Malik et al.
  • 1National Center for Livestock Breeding, Genetics & Genomics, PMAS Arid Agriculture University, Rawalpindi, 46300, Pakistan
  • 2Department of Animal Breeding & Genetics, Faculty of Veterinary & Animal Sciences, PMAS Arid Agriculture University, Rawalpindi, 46300, Pakistan
  • 3Animal Science Institute, National Agriculture Research Council, Islamabad, Pakistan
  • 4Department of Biotechnology, Faculty of Sciences, University of Sargodha, Sargodha, Pakistan
  • 5University Institute of Biochemistry & Biotechnology, PMAS Arid Agriculture University, Rawalpindi, 46300, Pakistan
  • *These authors contributed equally to this work.

Abstract. The identification issue of livestock can be resolved by using molecular identification tools that are acceptable to preserve and maintain pure breeds worldwide. The application of a molecular identification methodology is more important for developing nations, e.g., Pakistan, where uncontrolled crossbreeding has become a common practice and the import of exotic animals and germplasm is ever increasing. This presents a risk to local breeds as also stated by the FAO. Therefore, the current study was designed to develop standard molecular markers for Cholistani cattle to ascertain their purity for breeding purpose. In this study 50 and 48 unrelated males were sampled for Cholistani and each crossbred cattle, respectively. Candidate molecular markers present in Cholistani but absent in crossbred cattle and vice versa were detected using the amplified fragment length polymorphism (AFLP) method. Eleven markers were developed and were converted to single nucleotide polymorphism (SNP) markers for genotyping. The allele frequencies in both breeds were determined for discrimination ability using polymerase-chain-reaction–restriction-fragment-polymorphism (PCR-AFLP). The probability of identifying the Cholistani breed was 0.905 and the probability of misjudgment was 0.073 using a panel of markers. The identified markers can ascertain the breed purity and are likely to extend the facility for breed purity testing before entering into a genetic improvement program in the country.

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Identification issues in livestock can be resolved by molecular identification tools that preserve and maintain pure breeds worldwide. In this study, 50 and 48 unrelated Cholistani and crossbred males, respectively, were sampled. Candidate genetic markers present in Cholistani but absent in crossbred and vice versa were detected using the amplified fragment length polymorphism (AFLP) method. This study generated molecular breed-specific markers to identify the purity of the Cholistani breed.
Identification issues in livestock can be resolved by molecular identification tools that...
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