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Volume 61, issue 4
Arch. Anim. Breed., 61, 379-386, 2018
https://doi.org/10.5194/aab-61-379-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
Arch. Anim. Breed., 61, 379-386, 2018
https://doi.org/10.5194/aab-61-379-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Original study 02 Oct 2018

Original study | 02 Oct 2018

Association of single-nucleotide polymorphisms in NAT9 and MAP3K3 genes with litter size traits in Berkshire pigs

Jung Hye Hwang1,*, Sang Mi An1,*, Go Eun Yu1, Da Hye Park1, Deok Gyeong Kang1, Tae Wan Kim1, Hwa Chun Park2, Jeongim Ha1, and Chul Wook Kim1 Jung Hye Hwang et al.
  • 1Swine Science and Technology Center, Gyeongnam National University of Science & Technology, Jinju 660-758, South Korea
  • 2Dasan Pig Breeding Co., San 64-2, Gasan-ri, Eunbong-eub, Namwon-si 590-831, South Korea
  • *These authors contributed equally to this work.

Abstract. Litter size is an economically important trait in the pig industry. We aimed to identify genetic markers associated with litter size, which can be used in breeding programs for improving reproductive traits. Single-nucleotide polymorphisms (SNPs) of Berkshire pigs in the N-acetyltransferase 9 (NAT9) and Mitogen-activated protein kinase kinase kinase 3 (MAP3K3) genes were from RNA sequencing results, and already exist in the databank (NCBI), and were confirmed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). A total of 272 Berkshire sows were used to examine the genotype, and their association with litter size traits was analyzed. The NAT9 SNP was located in chromosome 12 exon 640 mRNA (A>G) and the MAP3K3 SNP was located in chromosome 12 intron 11 (80, C>T). Association analysis indicated that the GG genotype of NAT9 and the CT genotype of MAP3K3 had the highest values for litter size traits. The GG genotype expressed higher levels of NAT9 mRNA in the endometrium than the other genotypes did, and a positive correlation was found between litter size traits and NAT9, but not MAP3K3 expression level. These results indicate that the NAT9 and MAP3K3 can be used as candidate genes applicable in breeding program for the improvement of litter size traits in Berkshire pigs.

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Using RNA sequencing technology, we identified SNPs in the NAT9 and MAP3K3 genes that are associated with litter size in Berkshire pigs. Our results suggest that selecting Berkshire pigs with the GG genotype of NAT9 and the CT genotype of MAP3K3 can produce more piglets. We suggest that these genetic markers would improve litter size in breeding programs.
Using RNA sequencing technology, we identified SNPs in the NAT9 and MAP3K3 genes that are...
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