Journal cover Journal topic
Archives Animal Breeding Archiv Tierzucht
Journal topic

Journal metrics

Journal metrics

  • IF value: 1.203 IF 1.203
  • IF 5-year value: 1.203 IF 5-year 1.203
  • CiteScore value: 0.98 CiteScore 0.98
  • SNIP value: 0.920 SNIP 0.920
  • SJR value: 0.390 SJR 0.390
  • IPP value: 0.89 IPP 0.89
  • h5-index value: 11 h5-index 11
  • Scimago H index value: 23 Scimago H index 23
Supported by
Logo Leibniz Institute for Farm Animal Biology Logo Leibniz Association
Volume 61, issue 3 | Copyright
Arch. Anim. Breed., 61, 351-358, 2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Original study 03 Sep 2018

Original study | 03 Sep 2018

Evaluating bovine sperm transfection using a high-performance polymer reagent and assessing the fertilizing capacity of transfected spermatozoa using an in vitro fertilization technique

Ali Jafarnejad et al.
Related subject area
Subject: Livestock physiology | Animal: Cattle
Phenotypic analysis of milk coagulation properties and mineral content of Pinzgauer cattle breed
Carmen L. Manuelian, Mauro Penasa, Giulio Visentin, Martino Cassandro, and Massimo De Marchi
Arch. Anim. Breed., 61, 215-220,,, 2018
Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes
Vyankat Gangadhar Jadhav, Bagepalli Sathyanarayana Bharath Kumar, and Sujata Pandita
Arch. Anim. Breed., 61, 191-195,,, 2018
Milk fatty acid composition as an indicator of energy status in Holstein dairy cows
Lana Vranković, Jasna Aladrović, Daria Octenjak, Dušanka Bijelić, Luka Cvetnić, and Zvonko Stojević
Arch. Anim. Breed., 60, 205-212,,, 2017
Lipid and lipoprotein profile changes in dairy cows in response to late pregnancy and the early postpartum period
Francesca Arfuso, Francesco Fazio, Maria Levanti, Maria Rizzo, Simona Di Pietro, Elisabetta Giudice, and Giuseppe Piccione
Arch. Anim. Breed., 59, 429-434,,, 2016
Excessive amount of lactose in the diet of two-week-old calves induces urinary protein changes
Alicja Dratwa-Chałupnik, Małgorzata Ożgo, Adam Lepczyński, Agnieszka Herosimczyk, and Katarzyna Michałek
Arch. Anim. Breed., 59, 417-422,,, 2016
Cited articles
Alderson, J., Wilson, B., Laible, G., Pfeffer, P., and L'Huillier, P.: Protamine sulfate protects exogenous DNA against nuclease degradation but is unable to improve the efficiency of bovine sperm mediated transgenesis, Anim. Reprod. Sci., 91, 23–30, 2006.
Al-Shuhaib, M., Al-Saadi, A., Ewadh, M., and Noor, M.: Sperm Mediated Gene Transfer in Mammals, a Versatile Platform with Multiple Enhancements Techniques, J. Biol. Agric. Healthcare, 4, 58–68, 2014.
Anzar, M. and Buhr, M. M.: Spontaneous uptake of exogenous DNA by bull spermatozoa, Theriogenology, 65, 683–690, 2006.
Bachiller, D., Schellander, K., Peli, J., and Ruther, U.: Liposome-mediated DNA uptake by sperm cells, Mol. Reprod. Dev., 30, 194–200, 1991.
Brackett, B. G., Baranska, W., Sawichi, W., and Koprowski, H.: Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization, P. Natl. Acad. Sci. USA, 68, 353–357, 1971.
Short summary
In our study, we attempted to introduce a new device for optimizing sperm-mediated gene transfer. We utilized X-tremeGENE™ HP reagent from the Roche company in bovines, and one-third of total spermatozoa were transfected. We also examined the effect of DMSO on increasing the efficiency of the sperm/DNA transfer to oocytes via IVF. We examined these two reagents in bovine spermatozoa for the first time, which can be considered as the novelty of our work together with its originality.
In our study, we attempted to introduce a new device for optimizing sperm-mediated gene...