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Archives Animal Breeding Archiv Tierzucht
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Volume 61, issue 3
Arch. Anim. Breed., 61, 351-358, 2018
https://doi.org/10.5194/aab-61-351-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
Arch. Anim. Breed., 61, 351-358, 2018
https://doi.org/10.5194/aab-61-351-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Original study 03 Sep 2018

Original study | 03 Sep 2018

Evaluating bovine sperm transfection using a high-performance polymer reagent and assessing the fertilizing capacity of transfected spermatozoa using an in vitro fertilization technique

Ali Jafarnejad et al.
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Latest update: 10 Dec 2018
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Short summary
In our study, we attempted to introduce a new device for optimizing sperm-mediated gene transfer. We utilized X-tremeGENE™ HP reagent from the Roche company in bovines, and one-third of total spermatozoa were transfected. We also examined the effect of DMSO on increasing the efficiency of the sperm/DNA transfer to oocytes via IVF. We examined these two reagents in bovine spermatozoa for the first time, which can be considered as the novelty of our work together with its originality.
In our study, we attempted to introduce a new device for optimizing sperm-mediated gene...
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