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Volume 61, issue 3
Arch. Anim. Breed., 61, 321-328, 2018
https://doi.org/10.5194/aab-61-321-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
Arch. Anim. Breed., 61, 321-328, 2018
https://doi.org/10.5194/aab-61-321-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Original study 30 Jul 2018

Original study | 30 Jul 2018

Effect of Mucin13 gene polymorphism on diarrhoeagenic E. coli adhesion pattern and its expression analysis in native Indian pigs

Rebeka Sinha1,2, Nihar Ranjan Sahoo1, Kush Shrivastava1, Pushpendra Kumar1, Salauddin Qureshi3, Amit Kumar1, Gandham Venkata Papa Pydi Siva Ravi Kumar4, and Bharat Bhushan1 Rebeka Sinha et al.
  • 1Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India
  • 2Dairy Cattle Breeding Division, ICAR-National Dairy Research Institute, Karnal, Haryana – 132001, India
  • 3Standardization Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India
  • 4Veterinary Biotechnology Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India

Abstract. We identified genetic polymorphism in Mucin13 gene affecting E. coli adhesion patterns using (local isolate) diarrhoeagenic E. coli in Indian desi pigs. Five SNPs and one indel previously reported to be associated with enterotoxigenic E. coli (ETEC) F4ab/ac adhesion pattern were examined by designing PCR-RFLP protocol. The genotypic frequencies of only one SNP (g.22304A > G) differed significantly (at P ≤ 0.05) in adhesive, non-adhesive and weakly adhesive population. The AA (306sbp, 231bp), AG (306, 231, 108, 198bp) and GG (231, 198bp, 108b) genotypes of g.22304A > G locus were observed with frequencies 50.0%, 21.25% and 28.75%, respectively and AG genotype was significantly (P ≤ 0.05) associated with a non-adhesive pattern. The polymorphism information content of SNPs ranged from 17.67 (g.22124T > C) to 37.36% (g.21471C > T) loci. Three loci (g.21471C > T, g.22124T > C and g.22304A > G) were significantly departed from Hardy–Weinberg equilibrium. The linkage disequilibrium analysis revealed locus g.22124T > C and g.22304A > G were significantly (P ≤ 0.05) associated with each other. Expression profiling of target gene in jejuna of animals having AA, AG and GG genotypes revealed differences in various genotypes with the highest in the AA, moderate in the GG and low levels in the AG genotype, although they were statistically non-significant (at P ≤ 0.05). The absence of significant effect of genotypes on MUC13 mRNA expression indicates no direct functional role, although the structural role can not be ignored as the putative receptor gene is located within targeted genomic region. Further, reports of same SNP association with an ETEC F4ab/ac adhesion pattern indicate the target gene's role in diarrhoea even caused by other strains of E. coli which is not ETEC.

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We identified a genetic polymorphism in Mucin13 gene affecting E. coli adhesion patterns using (local isolate) diarrhoeagenic E. coli in Indian desi pigs. Five SNPs and one indel previously reported to be associated with an ETEC F4ab/ac adhesion pattern were examined by designing PCR-RFLP protocol. The genotypic frequencies of only one SNP (g.22304A > G) differed significantly (at P < 0.05) in an adhesive, non-adhesive and weakly adhesive population.
We identified a genetic polymorphism in Mucin13 gene affecting E. coli adhesion patterns using...
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