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Volume 61, issue 1 | Copyright
Arch. Anim. Breed., 61, 153-160, 2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Original study 28 Mar 2018

Original study | 28 Mar 2018

The novel T755C mutation in BMP15 is associated with the litter size of Iranian Afshari, Ghezel, and Shal breeds

Hamid-Reza Amini1,4, Amir Ajaki1,2, Majid Farahi1,2, Mitra Heidari1,2, Ahmad Pirali1,2, Mohsen Forouzanfar3, and Shahin Eghbalsaied1,2 Hamid-Reza Amini et al.
  • 1Transgenesis Center of Excellence, Isfahan (Khorasgan) branch, Islamic Azad University, Isfahan, Iran
  • 2Department of Animal Science, Faculty of Agriculture, Isfahan (Khorasgan) branch, Islamic Azad University, Isfahan, Iran
  • 3School of Sciences, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran
  • 4Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract. The present study was conducted to determine the molecular genetic variation in bone morphogenetic protein 15 (BMP15) of some selected Iranian sheep breeds and aims to provide relevant genetic information for twin-birth lambing. Therefore, a total of 44 rams and 213 ewes with single-, twin-, and triplet-birth lambing were used from Shal, Ghezel, Afshari and Lori-Bakhtiari sheep breeds. Three primer pairs were used for amplification of exons 1 and 2 of the BMP15 gene. The sequencing result of distinctive single-strand conformation polymorphism (SSCP) patterns showed segregation of the T755C mutation and the ensuing non-conservative substitution of L252P in the BMP15 propeptide. All three ewes with triplet-birth lambing and two sterile ewes were heterozygotic for this mutation. Association analysis revealed that this mutation was significantly related to the ewe's litter size, so that ewes with the CT genotype had 0.24 and 0.30 more lambs than those with the CC (p<0.05) and TT (p<0.01) genotypes, respectively. The mutation effect on the litter size was incomplete dominance in Afshari breed and over-dominance in Shal and Ghezel breeds. In conclusion, the findings in this experiment suggested synergistic effects of the T755C mutation and other unknown mutations in other effective genes which cause infertility and higher litter size in two different ways.

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Short summary
Birth of twins is partly controlled by genetic factors. Here, we genotyped ewes with triplet births, twin births, and single births as well as two infertile ewes from Iranian sheep breeds for the BMP15 gene. A new mutation was detected in two sterile ewes and all ewes with triplet-birth lambing. There is a relationship between the mutation and higher litter numbers. However, this mutation is not responsible for sterility or triplet births in Iranian sheep.
Birth of twins is partly controlled by genetic factors. Here, we genotyped ewes with triplet...