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Logo Leibniz Institute for Farm Animal Biology
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Arch. Anim. Breed., 60, 235-241, 2017
https://doi.org/10.5194/aab-60-235-2017
© Author(s) 2017. This work is distributed under
the Creative Commons Attribution 3.0 License.
Original study
25 Jul 2017
A novel synonymous SNP (A47A) of the TMEM95 gene is significantly associated with the reproductive traits related to testis in male piglets
Liangliang Liu1, Shuai Yu2, Rui Chen2, Xiaoyan Lv3, and Chuanying Pan2 1Department of Statistics and Information, School of Business Information, Shanghai University of International Business and Economics, Shanghai, 201620, P.R. China
2College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, P.R. China
3National Swine Foundation Seed Farm of Ankang Yangchen Modern Agriculture Group Co. Ltd, Ankang, Shaanxi, 725000, P.R. China
Abstract. Transmembrane protein 95 (TMEM95) is located on the acrosomal membrane of the sperm head involved in the acrosome reaction; thus, it is regarded as affecting spermatogenesis and reproduction traits. The aim of this study was to explore the novel single nucleotide polymorphisms (SNPs) within the pig TMEM95 gene as well as to evaluate their associations with the testicular sizes in male Landrace (LD) and Large White (LW) breeds. After pool sequencing and bioinformatics analysis, only one novel coding SNP was found in exon 1, namely NC_010454.3: g.341T > C, resulting in a synonymous mutation (A47A). This SNP could be genotyped using the StuI polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) assay. The minor allelic frequencies (MAFs) were 0.259 and 0.480 in the LD and LW breeds. Their polymorphism information content (PIC) values were 0.310 and 0.375. The LW population was at the Hardy–Weinberg equilibrium (HWE) (p > 0.05), whereas the LD population was not (p < 0.05). Association analyses demonstrated that a significant relationship was found between this A47A polymorphism and testis weight at 40 days of age in the LW population (p  =  0.047), and the heterozygote individuals showed lower testis weight than those with other genotypes. Moreover, this SNP was significantly associated with three testis measurement traits at 15 days of age in the LW population (p < 0.05); the individuals with genotypes TT and TC showed consistently superior testis measurement traits than those with genotype CC. These findings demonstrate that the A47A polymorphism had a significant effect on testis measurement traits, suggesting that the TMEM95 gene could be a candidate gene associated with reproductive traits. These results could contribute to breeding and genetics programs in the pig industry via DNA marker-assisted selection (MAS).

Citation: Liu, L., Yu, S., Chen, R., Lv, X., and Pan, C.: A novel synonymous SNP (A47A) of the TMEM95 gene is significantly associated with the reproductive traits related to testis in male piglets, Arch. Anim. Breed., 60, 235-241, https://doi.org/10.5194/aab-60-235-2017, 2017.
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Short summary
One novel coding SNP was found in exon 1 of pig TMEM95 gene, namely NC_010454.3:g.341T > C, which was significantly associated with testis measurement traits at 15 days of age in the LW population (p < 0.05). Moreover, a significant relationship was found between this A47A polymorphism and testis weight at 40 days of age in LW population (p = 0.047). These results could contribute to breeding and genetics programs in the pig industry via DNA marker-assisted selection (MAS).
One novel coding SNP was found in exon 1 of pig TMEM95 gene, namely NC_010454.3:g.341T  C, which...
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