Journal cover Journal topic
Archives Animal Breeding Archiv Tierzucht
Journal topic

Journal metrics

Journal metrics

  • IF value: 1.203 IF 1.203
  • IF 5-year value: 1.203 IF 5-year 1.203
  • CiteScore value: 0.98 CiteScore 0.98
  • SNIP value: 0.920 SNIP 0.920
  • SJR value: 0.390 SJR 0.390
  • IPP value: 0.89 IPP 0.89
  • h5-index value: 11 h5-index 11
  • Scimago H index value: 23 Scimago H index 23
Supported by
Logo Leibniz Institute for Farm Animal Biology Logo Leibniz Association
Volume 58, issue 2
Arch. Anim. Breed., 58, 433-439, 2015
https://doi.org/10.5194/aab-58-433-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.
Arch. Anim. Breed., 58, 433-439, 2015
https://doi.org/10.5194/aab-58-433-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.

Original study 26 Nov 2015

Original study | 26 Nov 2015

Effects of different cryoprotectants on the cryopreservation of cattle testicular tissue

S. Hu, Q.-C. Zhu, C. Han, X.-G. Zhang, B. Y. Song, D.-Q. Xie, S.-Y. Wei, and J.-H. Hu S. Hu et al.
  • College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi 712100, P.R. China

Abstract. Cryopreservation of testicular tissue is a new option in fertility preservation for prepubertal male animals. The purpose of this study was to explore the effects of different cryoprotectant agents (CPAs) at various concentrations on testes after the cryopreservation of calf testicular tissue. These experiments selected dimethyl sulfoxide (DMSO), glycerol, propylene glycol (PrOH), and sucrose as CPAs in varying doses (2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 %; v/v) in 8-month-old calf testicular tissue that was frozen and preserved. Then, cell viability, testosterone production, malondialdehyde (MDA) level, and superoxide dismutase (SOD) level were detected and analyzed following cryopreservation. The results showed that the optimal concentrations of DMSO, PrOH, glycerol, and sucrose were 10, 10, 7.5, and 10 %, respectively. Compared to the optimal concentrations of CPAs, cell viability and testosterone production decreased significantly at a lower and higher CPA concentration (P < 0.05). At the optimal concentrations of CPAs, the DMSO group showed higher cell viability and testosterone production than other CPA groups (P < 0.05). Compared to the optimal concentration of CPAs, the MDA level increased and the SOD level decreased at a lower or higher concentration of CPAs, but there was no significant difference (P > 0.05). Cell viability was significantly positively correlated with testosterone production (P < 0.05). In conclusion, DMSO provided the most effective protection for calf testicular tissue cryopreservation and the optimal concentration was 10 %.

Download
Citation
Share