Abstract. The study aimed at an assessment of a possibility of calves’ health status improvement in neonatal period by preventive application of 10 % ethanol extract of propolis (EEP). An influence of EEP on selected biochemical and haematological parameters of blood, body weight gains and diarrhoea symptoms intensity was determined. Propolis contains a range of biologically active compounds and exhibits numerous beneficial properties. Ethanolic extract is a form of propolis that is usually used in prevention. Forty five calves were used in the experiment. The assessment of clinical symptoms of diarrhoea, dehydration and vitality was conducted and calves (without symptoms of diarrhoea) were divided into 3 groups (15 calves in each): control, and two experimental (2 and 4 ml of EEP/day). The results of the study point that EEP may be a useful mean improving health status of calves. After an application of propolis in a dose of 4 ml/day higher daily gains were noted when compared to the control calves. Although no obvious influence of EEP on haematological parameters was noted, the positive influence on erythropoiesis and Fe content was observed. Higher EEP dose caused a significant decrease in lactic acid (LA) level. No influence on macroelements and electrolytes in blood serum was noted.