Articles | Volume 52, issue 2
https://doi.org/10.5194/aab-52-143-2009
https://doi.org/10.5194/aab-52-143-2009
10 Oct 2009
 | 10 Oct 2009

Development of eight-plex microsatellite PCR for parentage control in deer

A. Zsolnai, I. Lehoczky, A. Gyurmán, J. Nagy, L. Sugár, I. Anton, P. Horn, and I. Magyary

Abstract. Nine loci have been compiled into two multiplex microsatellite polymerase chain reaction (PCR) sets (four and five loci) and used as a tool to determine the most probable hind for each calf. The two sets were suitable to combine them in an eight-plex reaction. The exclusion probabilities of the eight-plex reaction and the nine loci were 99.3 and 99.6 % respectively, which allows the routine application of eight loci in wildlife management – as a first attempt to use molecular genetic information for such a task and it eliminates multiple sample handling in consecutive PCRs. Two loci out of the nine were never been used in deer previously.