Abstract. The objective of this study was to predict body weight (BW) of pre-slaughtering beef cattle using digital image analysis. Data used in this study were collected from slaughterhouses in Isparta and nearby provinces from 140 animals. Selected body measurements such as body weight (BW), wither height (WH), body length (BL), chest depth (CD), hip width (HW), hip height (HH) and body area (BA) of different breeds of beef cattle were combined and compared by digital image analysis. The body area was included as a different parameter for prediction of BW instead of chest girth. However, regression equation that included only body area gave the lowest R² value for Holstein (18.0%), but the R² value was 43.2 and 51.7% for Brown Swiss and crossbred animals, respectively. The regression equation which included all body traits resulted in R² values 35.3, 85.1, and 79.6% for Holstein, Brown Swiss and crossbred, respectively. The regression equation which included body area and body length showed that prediction ability of digital image analysis was high for prediction of BW in Brown Swiss and crossbred animals compared to Holsteins (R² 82.6, 76.5, and 29.5%, respectively). Results indicated that the prediction ability of digital image analysis was low for prediction of BW. Although possibility of using body area as a parameter in predicting BW is low it can be developed by further and better designed experiments.