Abstract. The studies were carried on 30 random 8-week old Swiss male mice. The mice of I group received peritoneally 100 μg/g b.w. of reduced glutathione (GSH) in 250 μl 0.9% NaCl , II group 200 μg/ g of GSH in 250 μl 0.9% NaCl and mice of III control group received 250 μl of 0.9 % NaCl. In the lysosomal fraction of the liver, kidney and the skeletal muscle the activity of nine lysosomal enzymes were estimated. GSH injections caused in the liver a statistically confirmed increase of activity of estimated hydrolases, only the activity LL decreased significantly and NAGL did not change. After injection of both GSH doses increased significantly the activity LAP, Cat. D and L, AP, BGAL, BGLU and NAGL in the kidney. After injection 200 μg/g of GSH increased significantly activity AAP but an activity EL and LL decreased. In the skeletal muscle was observed a statistically confirmed increase of AP, LL, BGAL and NAGL activity, only the BGLU and Cat. D and L (after 100 μg/g dose) activity decreased.